Cell and Gene Therapy Development

From a scientific concept to the development of novel therapies

At Eppendorf, our aim is to empower bioprocess experts in cell and gene therapy development to facilitate the translation of basic research into commercially viable applications. Leveraging decades of experience in upstream bioprocessing, and more than ten years of experience in bioreactor-based stem cell culture, we strive to advance cell cultivation, focusing on scalability, reproducibility, quality, time-to-market and cost-effectiveness.

Your expertise drives us, your challenges inspire us. Let's collaborate to find solutions that work for you.

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Supporting you in your pursuit of excellence in CGT

Standardize and scale up your cell culture

Stirred-tank bioreactors facilitate efficient cell expansion when the production of large quantities of cells in conventional 2D culture becomes too time-consuming and quality varies. Our solutions help you scale your process from flasks or bags to bioreactors and exploit the benefits offered by bioreactors in terms of scalability and process standardization.

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Discover our scalable bioreactor systems

Shorten time to market

Accelerate your time to market by leveraging all available data to identify critical process parameters for reproducible success. Discover how our digitalization and automation solutions can streamline routine tasks and expedite bioprocess development.

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Discover our bioprocess development solutions

Transition from R&D to manufacturing

Maintain consistency and quality as you scale up. Our scalable bioprocess solutions help you seamlessly translate your R&D successes into manufacturing. Our solution package of bioprocess controllers, bioreactors, bioprocess SCADA software, and services support the compliance of your bioprocesses with GMP requirements in clinical and commercial manufacturing.

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Discover our clinical and commercial manufacturing solutions

Be in control

When developing cell and gene therapies you need equipment that is reliably up and running. Support of compliance and professional documentation are critical in reducing the risks and time needed for audits. Find out how our service offerings help you being always in control.

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Upstream bioprocessing for various cell and gene therapy applications

Stem cell bioprocessing

When looking to scale up stem cell production, bioreactors stand as a powerful solution to establish reproducible expansion of stem cells. Learn more!

Viral vector production

Viral vector production is reliant upon thorough upstream bioprocess development. Read more about our bioprocess solutions!

Bioreactors suitable for scaling-up exosome production for cell and gene therapy development

Exosome/EV production

To explore the use of extracellular vesicles such as exosomes in cell and gene therapy development, large amounts need to be produced. Learn more about how bioreactors can help!

Get inspired by these resources on bioprocessing in CGT

Eppendorf bioprocess portfolio for CGT

Download flyer to get a quick overview on our bioprocess portfolio for cell and gene therapy development.

A Beginner's guide to cell therapy bioprocessing

This ebook is a must-read for everyone interested in bioprocessing in cell and gene therapy research. Download your copy now!

Bioprocessing resource guide

Find case studies and interviews demonstrating the huge potential of stirred-tank bioreactors for optimizing cell and gene therapy workflows.

Article collection

Learn more about recent advances in bioprocessing for cell and gene therapy applications.

How our customers use our products

Hannover Medical School: Increasing hiPSC yields

Increasing cell density is a vital component of research in stem cell bioprocessing. The team of Dr. Robert Zweigerdt from the Hannover Medical School, Germany used a DASbox^® Mini Bioreactor System to optimize their hiPSC bioprocess. In this application note they demonstrate how systematic process optimization led to a culture yield of 35 million hiPSCs per mL.

Download application note

iBET: Production of extracellular vesicles in bioreactors

Read, how scientists at the iBET, Instituto de Biologia Experimental e Tecnológica (iBET) implemented Raman spectroscopy to continuously monitor the glucose concentration, which has been identified as a critical process parameter for extracellular vesicle secretion.

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Ncardia® BV: Large-scale production of cardiomyocytes

Researchers from Ncardia^® BV developed a bioprocess for the large-scale manufacturing of cardiomyocytes derived from human induced pluripotent stem cell lines. Read, how they achieved reproducible cell growth and differentiation and how they scaled up the process from a DASbox^® Mini Bioreactor System into a BioFlo^® 320 bioreactor control system.

Download application note

Lentiviral vector production in packed-bed bioreactor

Read, how researchers at the University of Adelaide used a BioBLU^® 5p Single-Use Bioreactor filled with Fibra-Cel^® Disks for transient lentiviral vector production in adherent HEK 293T cells.

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iBET: Monitoring hiPSC expansion and differentiation

Read, how researchers at Instituto de Biologia Experimental e Tecnológica (iBET) used dielectric spectroscopy as a process analytical technology tool to monitor the expansion and differentiation of hiPSC aggregates in stirred-tank bioreactors.

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Généthon®: Scaling-down viral vector production

Read, how researchers at Généthon® developed a scale-down model for rAAV viral vector production in Sf9 cells using an Eppendorf DASbox^® Mini Bioreactor System.

Download application note

Kadimastem: Stem cell bioprocessing

Kfir Molakandov, PhD, is the Head of Diabetes Cell Therapy Department at Kadimastem in Ness Ziona, Israel. In this interview, he talks about stem cell bioprocessing and current challenges in PSC-derived pancreatic islets therapy.

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Join the stem cell community

The years have taught us that the real expert is you. This is why we organize the Stem Cell Community Day (SCCD) since 2017. It connects researchers from academia and industry across the world to discuss recent advances and new technologies in the field of stem cell production with a special focus on cultivation in stirred-tank bioreactors.

Let’s talk innovation, let’s talk progress!

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Cell and Gene Therapy Development

From a scientific concept to the development of novel therapies

Supporting you in your pursuit of excellence in CGT

Standardize and scale up your cell culture

Shorten time to market

Transition from R&D to manufacturing

Be in control

Upstream bioprocessing for various cell and gene therapy applications

Stem cell bioprocessing

Viral vector production

Exosome/EV production

Get inspired by these resources on bioprocessing in CGT

Eppendorf bioprocess portfolio for CGT

A Beginner's guide to cell therapy bioprocessing

Bioprocessing resource guide

Article collection

How our customers use our products

Hannover Medical School: Increasing hiPSC yields

iBET: Production of extracellular vesicles in bioreactors

Ncardia® BV: Large-scale production of cardiomyocytes

Lentiviral vector production in packed-bed bioreactor

iBET: Monitoring hiPSC expansion and differentiation

Généthon®: Scaling-down viral vector production

Kadimastem: Stem cell bioprocessing

Join the stem cell community